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Chemoproteomic Identification i r e f B12-ABP Binding Proteins, Competitive Inhibition Studies, and I r e f Proteomic Analysis of Halomonas HL-48. LC-MS Proteomic Measurement and Quantitative Characterization of Halomonas Proteins Captured by the B12-ABP.

Gene Cloning and Purification of Recombinant PhrR, FolD, MetE, and MetH Proteins. Fluorescence Gel Analysis of B12-ABP Labeling of Purified FolD, MetE, MetH, and PhrR Proteins.

Genomic Reconstruction of PhrR Regulons. Measurement of Intracellular Folate and Derivatives. SI Materials and MethodsChemicals and F for Synthesis. General Methodology for Synthetic Chemistry. B12-ABP Labeling and CNB12 Competition Studies of Pure Proteins for Fluorescence Gel Analysis, and HL-48 Proteins for LC-MS Proteomic Analyses. Analytical Processing of LC-MS Data. Gene Cloning and Purification of Recombinant PhrR Protein. Gene I r e f and Purification of Recombinant FolD, MetH, and MetE Proteins.

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